TABLE 1.
Oligonucleotide primers or probes used in these studies
| Target | Primer or probe | Sequence (5′-3′) |
|---|---|---|
| incA | CT119F | ACATGTAAGCTGGTTTTTACATAG |
| CT119R | TGGCACCCTGTGTACAGTTAG | |
| omp1 | 681F | CTTTAACTAGGACGCAGTGCCGC |
| 681R | TGGACCCGACCGAAGCCGAGCC | |
| 81F2 | CTGAGATGTTTACAAATGCCGC | |
| U681R3 | AGACCATTTAACTCCAATGTA | |
| tarp | TPF1 | TTCTCCAGATACTTCAGAAAGC |
| TPF2 | CAAAGACTCTGACGGAGCTGGTG | |
| TPR1 | TGCACACATGTTTTCCAAGTC | |
| TPR2 | CAGGGTAAACGACGTCTAGG | |
| TPR3 | CCTCCAGGAATTCGTCCTCCG | |
| CT166 | CT166F | CTCCTCCAGAAGGAACGACAAC |
| CT166FA | CTTCAGAGAATAAGGTCACTAC | |
| CT166F2 | AAGAAAAACCTAAGACGACTCCG | |
| CT166F3 | AACGCGTGCAGAGTATTTAGAG | |
| CT166R | CCCAGCATACATGTCTCCATC | |
| incA RT-PCR | RT-PCR incA F | CACATTAGCAGGGAATGCTC |
| RT-PCR incA R | AGAGCCTTTAAGATCTGC | |
| incA | RCE1 | CTTTTTGTAGAGGGTGAT |
| incA Q-PCRa | Q-PCR incA F | AACTTTAAATGAGAAAGTGCCTATGACAA |
| Q-PCR incA R | ATCAGAAAGCCAACAAGATGT | |
| incA probeb | TATTTTCTTAATTTGTCCATCAAAGAA | |
| groEL Q-PCR | Q-PCR groEL F | TGTTACCGTTGCGAAAGAAGT |
| Q-PCR groEL R | TCCAGCTTTGTCAGCAGTTT | |
| groEL probeb | ACATGAAAATATGGGCGCTC |
a
Q-PCR, quantitative PCR.
b
Quantitative PCR probes are labeled with 6-carboxyfluorescein on the 5′ end and 6-carboxytetramethylrhodamine on the 3′ end.