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Expression of aniA by M. catarrhalis ETSU-9 wild-type and mutant strains under different growth conditions, as measured by qRT-PCR. Total RNA was isolated from wild-type ETSU-9 (wt) and ETSU-9 nsrR mutant (nsrR) cells grown in the planktonic state (PT) under iron limitation conditions in broth containing 30 μM Desferal (DF30) and in a continuous-flow biofilm system (BF) as described previously (62). qRT-PCR was performed as described in Materials and Methods.
