FIG. 5.

Identification of M. catarrhalis genes regulated by NsrR. (A) A DNA microarray analysis of total RNA isolated from wild-type strains O35E, 7169, and ETSU-9 and nsrR mutants of these strains was performed as described in Materials and Methods. Data for the genes consistently upregulated at least twofold (P < 0.002) in all three nsrR mutants are shown. The error bars indicate standard deviations. (B) qRT-PCR analysis of the relative levels of expression of norB, aniA, MC ORF 683, and MC ORF 1550 in nsrR mutant and wild-type cells of strain O35E. The endogenous control for the qRT-PCR measurements was expression of MC ORF 1234 (62). The maximum and minimum relative levels of expression are indicated by the error bars.