FIG. 6.

Effects of nitrogenous compounds on expression of genes regulated by NsrR. (A and B) Total RNA extracted from cells of M. catarrhalis wild-type strain O35E (A) and the O35E aniA mutant (B) grown in the presence or absence of 5 mM NaNO₂ were used for qRT-PCR. (C) Total RNA extracted from logarithmic-phase M. catarrhalis O35E cells grown for 90 min in the presence or absence of the NO-generating compound spermine NONOate was used for qRT-PCR. The relative levels of expression of norB (bars 1), aniA (bars 2), MC ORF 683 (bars 3), and MC ORF 1550 (bars 4) are shown. The endogenous control for the qRT-PCR measurements was the expression of MC ORF 1234 (62). The maximum and minimum relative levels of expression are indicated by the error bars.