TABLE 2.
Oligonucleotide primers used in this study
| Primer | Sequence (5′-3′) |
|---|---|
| WW207 | TATCCCGGGTTCTCCTTATAGAAAGGT |
| WW210 | GACACGCCCATCACAGACTAA |
| WW211 | GCAACTCAACATGCTCATGAT |
| WW216 | TTGGATCCGCGCACTGTCAACGACTTTa |
| WW217 | GACAATCCATTTGCCCGGGTGGTTTGAGAACTGCACTb |
| WW218 | GGCAAATGGATTGTCTGGTAA |
| WW219 | CGACGAGCTCCCATTCTGCAATCTTAGGTc |
| WW220 | GAGGATCCACCACTTATATTTCACAa |
| WW221 | GTTTTAGATTGCCCGGGTCAATGATGGCTGGATTTAb |
| WW222 | CGGGCAATCTAAAACACTCAATCTCA |
| WW223 | TGGCGAGCTCCATATCAAAGGGCTGTAGAc |
| WW247 | TAGGATCCAATCACACTTAGGATTATCA |
| 683Fw | GGACATGATGGGCATGGTC |
| 683Rv | TAAGGCAGGTGCTGGCG |
| 1550Fw | AGACACCATCAGCGTTGAGCT |
| 1550Rv | AAGGTGACCTCTTCGGTGACAT |
a
The BamHI site is underlined.
b
The SmaI site is underlined.
c
The SacI site is underlined.