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. 2008 Sep 12;190(23):7719–7727. doi: 10.1128/JB.01573-07

FIG. 2.

FIG. 2.

Growth curves and Northern blot analyses for the virR mutant (TS133) of C. perfringens. (A) Growth curves for C. perfringens strains with a 1% inoculum. All three C. perfringens strains grew with a doubling time of ∼20 min in Gifu anaerobic medium. ○, strain 13(pJIR418); □, strain TS133(pJIR418) (virR); ▵, strain TS133(pBT405) (virR+ virS+). OD 600 nm, optical density at 600 nm. (B) Total RNA was prepared from each culture at the indicated times (2 and 3 h). Either 10 μg (for virT and ccp) or 40 μg (for virU) of total RNA was resolved by agarose electrophoresis, blotted onto a nylon membrane, and hybridized with probes for virT (CPE0845), ccp (CPE0846), and virU (CPE0920). Lane 1, strain 13(pJIR418); lane 2, strain TS133(pJIR418); lane 3, strain TS133(pBT405).