TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| C. perfringens strains | ||
| 13 | Wild-type strain (type A) | 14 |
| TS133 | Strain 13 virR::Tetr | 24 |
| TS140 | Strain 13 Δvrr Emr | 28 |
| TS190 | Strain 13 virT::Emr | This study |
| E. coli DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Takara Bio Inc. |
| Plasmids | ||
| pBT405 | pJIR418 Ω(PstI 4.3-kb strain 13 genomic library) (virR+virS+ complementation vector), Ampr | Shimizu, unpublished data |
| pJIR418 | E. coli-C. perfringens shuttle vector, Cmr Emr | 29 |
| pSB1031 | pJIR418 Ω(PCR-amplified 637-bp fragment) (vrr+ complementation vector) | 4 |
| pTS930 | pJIR418 Ω(PCR-amplified 1,400-bp fragment) (virT+ complementation vector) | This study |
| pTS931 | pJIR418 Ω(PCR-amplified 539-bp fragment) (virU+ complementation vector) | This study |
| pTS932 | pTS930 (585T→A) | This study |
| pTS933 | pTS931 (182A→T) | This study |
| pTS934 | pUC118 Ω(PCR-amplified 334-bp fragment) (virT+ suicide vector), Emr | This study |
| pTS935 | pTS930 (1-bp frameshift at position 33) | This study |
| pTS936 | pTS931 (1-bp frameshift at position 15) | This study |
| pUC19 | Cloning vector, AmprlacZ′ pMB 1 ori | Takara Bio Inc. |
| pUC118 | Cloning vector, AmprlacZ′ pMB 1 ori | Takara Bio Inc. |
a
Tetr, resistance to tetracycline; Emr, resistance to erythromycin, Cmr, resistance to chloramphenicol; Ampr, resistance to ampicillin.