TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence (5′to 3′) | Use |
|---|---|---|
| virT-F | GAAGGTAAGTACCAAGATGA | Northern blotting |
| virT-R | TGATATTGCCACCCCAACTT | Northern blotting |
| ccp-F | GAGGCCGAAAAGACTGAAGG | Northern blotting |
| ccp-R | GCCCAATGTGGTATTGCTTG | Northern blotting |
| virU-F | CGATTCGTTTTTGATAGAAATGG | Northern blotting |
| virU-R | TTTATTTCTAGTTTTTCCTTTGATGA | Northern blotting |
| vrr-F | GACCAGTTACGCACAAAC | Northern blotting |
| vrr-R | GGACAGTTCTATTTCTAGG | Northern blotting |
| pfoA-F | GCAAGTATTGCAATGGCTTT | Northern blotting |
| pfoA-R | GTAAGTAATACTAGATCCAGGGT | Northern blotting |
| colA-F | GGTCTAGAGGATAGTGGAAGA | Northern blotting |
| colA-R | GTTCCTCTCATATCGTAAGT | Northern blotting |
| 0845-PE | TTTATCAATGGGTAACGTAAAGAAAGTACCAGATA | Primer extension |
| 0846-PE | GTTGAAACTCCTCCTAATAGAACGCTACCAATGGT | Primer extension |
| 0920-PE | TCGGTATAATAAAAATGATATTGTTACTAATATG | Primer extension |
| virT-NM | GAAAGTACCAGATTAAATAGAAATAG | Site-directed mutagenesis |
| virU-NM-1 | TTGGTATTATATATTAATATTTTCGTC | Site-directed mutagenesis |
| virU-NM-2 | GACGAAAATATTAATATATAATACCAA | Site-directed mutagenesis |
| virT-FM-F | ATCGCTTTAAGTTTATCATTTCTATTTTATCTGGT | Site-directed mutagenesis |
| virT-FM-R | ACCAGATAAAATAGAAATGATAAACTTAAAGCGAT | Site-directed mutagenesis |
| virU-FM-F | GATATGAAAGACGAAAAATTAATATAAAATACCAA | Site-directed mutagenesis |
| virU-FM-R | TTGGTATTTTATATTAATTTTTCGTCTTTCATATC | Site-directed mutagenesis |