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. 2008 Sep 26;190(23):7621–7632. doi: 10.1128/JB.00806-08

FIG. 1.

FIG. 1.

Growth characteristics of the S. epidermidis strain 1457 aconitase mutant. (A) Aconitase activity of S. epidermidis strains 1457, 1457-acnA, and 1457-acnA containing pMRS2 (1457-acnA + pMRS2) was determined 6 h postinoculation. (B) Growth curves of wild-type strain 1457, aconitase mutant strain 1457-acnA, and strain 1457-acnA containing the complementing plasmid pMRS2 grown in TSB containing 0.25% glucose. At the indicated times, aliquots were removed, and the optical density at 600 nm (O.D.600) was measured, and the pH of the culture medium was determined. (C) Temporal depletion of glucose from the culture medium of strains 1457, 1457-acnA, and 1457-acnA containing pMRS2. (D) Temporal accumulation and depletion of acetic acid in the culture medium of strains 1457, 1457-acnA, and 1457-acnA containing pMRS2. The results are representative of at least two independent experiments.