FIG. 3.

Northern blot analysis of UDP-N-acetylglucosamine biosynthetic genes in S. epidermidis strains 1457 and 1457-acnA. At the indicated times during the growth cycle, total RNA was isolated from strains 1457 and 1457-acnA, and Northern blot analysis was performed using the indicated probes. To ensure that equivalent quantities of RNA were loaded, 23S and 16S rRNA were visualized by ethidium bromide staining and used as loading controls (bottom panel). The results are representative of at least two independent experiments.