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Aconitase inactivation increases transcription of icaR, sarA, and sigB. Northern blot analysis with icaR, sarA, and sigB with DNA probes generated using primers shown in Table 2. To ensure that equivalent quantities of RNA were loaded, 23S and 16S rRNA were visualized by ethidium bromide staining and used as loading controls (bottom blot). The results are representative of at least two independent experiments.
