FIG. 8.

Partial biochemical inhibition of aconitase increases PIA synthesis in sigB and icaR mutants. Bacterial cultures were incubated with 400 μM fluorocitric acid and grown for 2 h, and the relative amounts of cell-associated PIA were determined by a PIA immunoblot assay. The data are presented as the means plus standard errors of the means (error bars) from three independent experiments. Values that were significantly different (P < 0.05) from the value for the isogenic untreated control (0 μM fluorocitrate) are indicated by an asterisk.