TABLE 1.

Bacterial strains, bacteriophage, and plasmids used in this study

Bacterial strain, bacteriophage, or plasmid	Relevant propertiesa	Source or reference
Bacterial strains
DH5α	E. coli cloning host	Invitrogen
RN4220	S. aureus restriction-deficient strain 8325-4	49
1457	S. epidermidis biofilm-forming strain	43
1457-acnA	Strain 1457 acnA inactivated mutant; Minr	This study
1457-citZC	Strain 1457 citCZ deletion mutant; Ermr	This study
1457-Δica	Strain 1457 icaADBC deletion mutant; Tmpr	26
1457-icaR	Strain 1457 icaR inactivated mutant; Minr	26
1457-sigB	Strain 1457 sigB inactivated mutant; Tmpr	26
1457-sarA	Strain 1457 sarA inactivated mutant; Minr	26
Bacteriophage 71	S. epidermidis transducing phage	15
Plasmids
pCL15	Expression vector; derivative of pSI-1; Camr	Chia Lee
pEC4	pBluescript II KS(+) with ermB inserted into ClaI site; Ampr Ermr	5
pJF12	Plasmid pCR2.1 containing tetM; Ampr Minr	J. Finan and G. Archer
pGEM-T	E. coli TA cloning vector; Ampr	Promega
pMRS2	pCL15 with acnA gene under the control of the Pspac promoter; Camr	This study
pMRS5	Derivative of pTS1-d with citZC::ermB fragment; Ampr Camr Ermr	This study
pNF103	pUC19 with 1.7-kb internal fragment of acnA; Ampr	This study
pNF117	pNF103 with tetM inserted into EcoRI site; Ampr Minr	This study
pNF118	pRO6448::pNF117; Ampr Ermr Minr	This study
pRO6448	pE194(ts) with AluI fragment (oriT) of pC221 cloned into the unique ClaI site; Ermr	51
pTS1	Shuttle vector; pE194orits; ColE1; Ampr Camr	24
pTS1-d	Derivative of pTS1 with deletion of plasmid-encoded 3′ region of ermC	This study
pUC19	E. coli cloning vector; Ampr	83

^aAmp^r, ampicillin resistant; Cam^r, chloramphenicol resistant; Erm^r, erythromycin resistant; Min^r, minocycline resistant; Tmp^r, trimethoprim resistant; ts, temperature-sensitive origin of replication.