TABLE 2.
Oligonucleotide primers used in this study
| Primer | Nucleotide sequence (5′→3′) |
|---|---|
| 663 forward | GCGCGCGGCGCCGGGATATAAAATGGCTTCTA |
| 664 reverse | GCGCGCCTGCAGACCAGCAAGCGCATAAGCTA |
| SERP1762(glmM)-f | CACCTGAATTGGCTTTTAAACTAGG |
| SERP1762(glmM)-r | TCAACACGATATTTGTCAGTCACTC |
| SERP1760(glmS)-f | GTGTGGAATTGTTGGTTATATTGGC |
| SERP1760(glmS)-r | GCTTCACGTGATAATGTTGAACC |
| glmU-f | CGATTATTCTGGCAGCAGGTAAG |
| glmU-r | CGATTGATACGTTGTTGCAAAGC |
| icaProbeforward | GACAGTCGCTACGAAAAG |
| icaProbereverse | CCGAATAATTTGTAAATTTCC |
| icaR-r | GGAGGCTCTAAAATACCAAGTCTC |
| icaR-f | GGTACGATGGTACTACACTTGATG |
| pgi-f | GCATGAACTAGATCAGCAAAAGGATATTG |
| pgi-r | GCTGTAAGAACAGAATAACGACCTCC |
| sarA-f | GGCTATTTCAAAAATCAATGACTGCTTTG |
| sarA-r | GCTTCTGTGATACGGTTGTTTACTC |
| sigB-f | CGAAAGAGTCGAAATCAGCTAGTG |
| sigB-r | GTGAAACATGCATTTGACTAAGACC |
| SalI-SD-acnA-f | CTAAGTCGACAATATCAAGGGGGATATAAAATGGCTTC |
| SacI-acnA-r | CAAGAGCTCATGACTG CTTGTACTCAATAGTGAG |
| pCL15-f | GTTCTACATCCAGAACAACCTCTGC |
| pCL15-r | GAAATGATGACCTCGTTTCCACCG |
| citC-r-ermB2 | GACGTTCTGTACCTTCTTTAGGCGACTCATAGAATTATTTCCTCCC |
| citZ-f-ermB | CACTTTGAGGAGTATGTGACGAAGGAGGGATTCGTCATGTTG |
| phoP-BamHI | GGaGGATCCACGTACAGTCTTTATCAATTTAGGTTGC |
| ermB-citC-f2 | GGAAATAATTCTATGAGTCGCCTAAAGAAGGTACAGAACGTCTAGTAAGAG |
| ansP-SacI | GCAGAGCTCACTAAGTGTCGATGTAAATTTTATCGTGC |
| ermB-citZ-r | GACGAATCCCTCCTTCGTCACATACTCCTCAAAGTGCTTGTATAC |