TABLE 4.
Summary of metabolic differences identified in S. epidermidis strains by NMR after 6 h of growth
| Increased intracellular concn in the wild-type strain 1457a | Increased intracellular concn in strain 1457-acnAa | Similar intracellular concns in wild-type and 1457-acnA strains |
|---|---|---|
| Glutamine | Ethanol | Glucosamine-6-phosphate |
| Glutamate | Alanine | Glucose |
| Ornithine | Lactate | Ribose |
| Succinate | Acetate | Glucose-6-phosphate |
| Asparagine | Acetaldehyde | NAD |
| Aspartate | Citrate | UDP-glucuronate |
| γ-Aminobutanoate | UDP-N-acetylglucosamine | Ribose-5-phosphate |
| Isocitrate | Uridine | Inosine |
| Fructose-6- | Adenosine | |
| phosphate | Xanthosine |
a
The intracellular concentration was considered to be increased when the percent difference in the NMR peak intensities was fivefold greater than the percent error observed in the peak intensities between the triplicate NMR spectra.