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LC-MS analysis of LamD558 production in the culture supernatants of wild-type and mutant strains of L. plantarum. The overnight culture supernatant of each strain was partially purified by using a Sep-Pak C18 cartridge column and then analyzed by LC-MS as described in Materials and Methods. Extracted ion chromatograms were plotted with detector counts at m/z 559, corresponding to the protonated molecular ion of LamD558.
