FIG. 5.

Kinetics of gene expression: ratios of the wild-type strain to the ΔlamA, ΔlamR, and ΔlamA ΔlamR mutants. Cell cultures grown in MRS at 37°C without agitation were collected in the early, middle, and late logarithmic growth phases. Then total RNA was isolated and cDNA synthesis was performed as described in Materials and Methods. The vertical line indicates the relative gene expression level compared to expression of the control lactate dehydrogenase gene. All the Q-PCR analyses were performed in triplicate. ▪, wild-type strain; □, ΔlamA mutant; ▴, ΔlamR mutant; ▵, ΔlamA ΔlamR mutant.