TABLE 2.
Primers used for construction of deletion plasmids and Q-PCR analysis
| Primer | Sequence (5′-3′) | Remarks |
|---|---|---|
| lamR-XbaI | TCTAGAATTGGGACTGGTGGTGCTGTTTAC | Deletion plasmid construction |
| lamR-SmaI1 | CCCGGGAGACGGCTTGAGTTGGTTGCTGAC | Deletion plasmid construction |
| lamR-SmaI2 | CCCGGGATTTAGATGATGGTGAACTTGA | Deletion plasmid construction |
| lamR-BamHI | GAGCTCAAATAATCTTGTAATGCCTCTTG | Deletion plasmid construction |
| lamR-F | CAAGTGCTTAATGGGGTAA | Deletion plasmid construction |
| lp_930-F | CACGGGTGGCGGATAAGGTT | Alkaline shock protein |
| lp_930-R | TGTCATTTTGTTGCCGCCACTC | |
| lp_1197-2F | GACAAGCACGACCAACTCACA | Cps2A |
| lp_1197-2R | TAAGCCGACAACTAGCCCAATCAA | |
| lp_1204-F | ACGTCATCCATTTCGCTTTTT | Cps2H |
| lp_1204-R | GTCTCATTCCACGCATCTCTGT | |
| lp_3087-F | TATTAGCGCATGTCAAAAGTCAGC | LamR |
| lp_3087-R | CTCCGTATGCGTCGTCACAAAGAT | |
| lp_3088-F | TGAGCGCCATTTTTGTTACG | LamK |
| lp_3088-R | GCCCCGCTCTTGTCCCTTAG | |
| lp_3414-F | TGGGACGCATACGCAACCTAA | Extracellular protein |
| lp_3414-R | GCCAGCCAGCACCAGTCC | |
| lp_3575-F | CGCGTTTCAGATTGCCTTTTT | Integral membrane protein |
| lp_3575-R | CGTGCCAATCTTCCCAACAATA | |
| lp_3578-F | AAGCCGGTTGTTCCAGTTCAT | Catalase |
| lp_3578-R | GGCATCCGGCACCTCTTCT | |
| lp_3579-F | GCATGCCAAGATCCCCACGAA | Negative regulator of proteolysis |
| lp_3579-R | TCCGGCCCATGTCACGAA | |
| lp_3581-F | GATTGGCCTATTTGTCATT | LamC |
| lp_3581-R | GAGCTTCGATTTCATTCA | |
| lp_3582-F | CACCGGCTGTTACAAGAAAGAA | LamB |
| lp_3582-R | AAACTAATAACAGCGATACAACAA | |
| lp_3583-F | GATGGGCGCTTAACGGATGGAC | ATP-dependent protease subunit ClpL |
| lp_3583-R | TCTGGGCGGAAAAATGGTGCTAA | |
| LDH-F | TGATCCTCGTTCCGTYGATG | Lactate dehydrogenase |
| LDH-R | CCGATGGTTGCAGTTGAGTAAG |