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Germination of sleL spores is slightly delayed. Heat-activated spores were germinated in BHI medium at 39°C. Germination and outgrowth of wild-type (•) and DPBa27 (sleL-lacZ::pDPV350) (▪) B. anthracis spores were followed as changes in OD600 value over time. Error bars represent 1 standard deviation of the mean of three independent assays. The difference in the loss of OD600 between the two strains is significant (P ≤ 0.0098) from 10 to 35 min after germination initiation.
