TABLE 2.
Muropeptide peak identification
| Muropeptide | Structurea |
|---|---|
| Produced from dormant spore peptidoglycan | |
| A | DS-TriP |
| B | DS-Ac-TriP |
| C | DS-TriP+Am |
| D | DS-Ac-TriP+Am |
| E | DS-Ala |
| F | DS-TP |
| G | DS-Ac-TP |
| H | TS-TP open lactam |
| I | TS Red-TP |
| J | TS Red-Ala |
| K | DS-Ac-TP × TriP+Am-DS-Ac |
| L | TS-TP-Ac |
| M | DS-Ac-TP+Am × TriP+Am-DS-Ac |
| N | TS-TP |
| O | TS-TP × TP |
| P | DS-TP × TP-TS Red |
| Q | TS-Ala |
| R | HSRed-Ac-TP (right lactam reduced) |
| S | HSRed-Ac-TP (left lactam reduced) |
| T | DS-TP × TP-TS |
| U | HS-TP-Ac |
| V | TS-TP × TP-TS |
| W | HS-Ala-Ac |
| X | HS-Ala-Ac |
| Y | HS-TP |
| Z | HS-Ala |
| AA | TS-TP × TP-HS |
| Produced from germinated spore exudate following mutanolysin digestion | |
| aG1 | TriS-TP Red |
| aG4 | TriS-TP |
| aG5 | TriS-Ala |
| aG8 | PS-TP-Ac |
| Produced from germinated spore exudate with no mutanolysin digestion | |
| aG2 | TS-TP NAGr Red |
| aG3 | TS-Ala NAGr Red |
| aG6 | TS-TP NAGr |
| aG7 | TS-Ala NAGr |
| aG7a | TS-TP anhydro |
| aG7b | TS-Ala anhydro |
| aG10u | HS-TP-Ac NAGr |
| aG12 | HS-Ala-Ac NAGr |
a
DS, disaccharide (NAG-NAM); TS, tetrasaccharide (NAG-lactam-NAG-NAM); HS, hexasaccharide (NAG-lactam-NAG-lactam-NAG-NAM); TriS, trisaccharide (lactam-NAG-NAM); PS, pentasaccharide (lactam-NAG-lactam-NAG-NAM); TriP, tripeptide (Ala-Glu-Dpm); TP, tetrapeptide (Ala-Glu-Dpm-Ala); -Ac, deacetylated glucosamine; +Am, amidated Dpm; Red, reduced lactam (an artifact of sample preparation [47]); NAGr, NAG at the reducing end; ×, cross-link between two peptides; anhydro, anhydro form of NAM at the reducing end.