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Functionalization of surfaces with HBHA and actin. AFM topographic images (5 μm by 5 μm) recorded in PBS for NTA/EG-terminated supports after functionalization with HBHA having their N-terminal (A) or C-terminal (B) ends exposed, and for NHS/EDC-terminated supports after functionalization with actin (C). A small area was first scanned at large forces and high rates, followed by imaging of a larger image of the same region under small forces.
