FIG. 2.

Force spectroscopy of the HBHA-actin interaction. (A and C) Schematics of the surface chemistry used to functionalize model supports with actin and AFM tips with HBHA having their N-terminal (N-term) (A) or C-terminal (C-term) (C) ends exposed. (B and D) Representative force curves and adhesion force histograms (n = 512) recorded between actin and either the N-terminal domain (B) or the C-terminal domain (D) of HBHA. All curves were obtained using a retraction speed of 1,000 nm s⁻¹ and an interaction time of 500 ms.