FIG. 3.

Dependence of the HBHA-actin interaction force on the pulling speed. Adhesion force histograms obtained in PBS between actin and HBHA having either their N-terminal (A) or C-terminal (B) ends exposed, using a pulling speed of 100 nm s⁻¹, 500 nm s⁻¹, 1,000 nm s⁻¹, 5,000 nm s⁻¹, and 7,000 nm s⁻¹. Constant interaction time (500 ms) and approach speed (1,000 nm s⁻¹) were used.