Multiple alignment of HBHA with different actin-binding proteins. Only the domains homologous to HBHA are shown. Residues identical between at least two aligned proteins are indicated in white in either black or gray boxes. Negative (−) and positive (+) charges conserved among at least three aligned proteins are indicated. Accession numbers for tropomyosin 1 (TPM1), ezrin 1 (EZR1), and myosin 9 (MYO9) are NP_001018007.1, P15311, and P35579, respectively.