TABLE 1.
List of strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli strains | ||
| E2348/69 | EPEC wild type | J. Kaper |
| TU1403 | E2348/69 carrying a chromosomal ler-His6 | 3 |
| DF2 | E2348/69 ler::kan | 12 |
| A70 | CC102 (ara Δ(lac-proB)XIIIthi F′-lacI378 lacZ461 proA+B+) mutS215::Tn10 | S. Altuvia |
| W3110 | F− λ−thyA36 deoC2 IN(rrnD-rrnE)1 | A. Oppenheim |
| Plasmids | ||
| pIR1 | pKK177-3 derivative containing gfpmut3 | 12 |
| pGY1 | pIR1 containing a transcriptional fusion of PLEE1, ler, and gfpmut3 (PLEE1-ler-gfpmut3) | This study |
| pGY2180 | pGY1 carrying PLEE1-ler(L16P)-gfpmut3 | This study |
| pGY2206 | pGY1 carrying PLEE1-ler(L23R)-gfpmut3 | This study |
| pGY2189 | pGY1 carrying PLEE1-ler(L56P)-gfpmut3 | This study |
| pGY2190 | pGY1 carrying PLEE1-ler(Y77H)-gfpmut3 | This study |
| pGY2191 | pGY1 carrying PLEE1-ler(Y77C)-gfpmut3 | This study |
| pGY2192 | pGY1 carrying PLEE1-ler(G82V)-gfpmut3 | This study |
| pGY2207 | pGY1 carrying PLEE1-ler(G82E)-gfpmut3 | This study |
| pGY2208 | pGY1 carrying PLEE1-ler(W85R)-gfpmut3 | This study |
| pGY2209 | pGY1 carrying PLEE1-ler(G87S)-gfpmut3 | This study |
| pGY2210 | pGY1 carrying PLEE1-lerG87D)-gfpmut3 | This study |
| pGY2211 | pGY1 carrying PLEE1-ler(G89D)-gfpmut3 | This study |
| pGY2193 | pGY1 carrying PLEE1-ler(P92S)-gfpmut3 | This study |
| pGY2194 | pGY1 carrying PLEE1-ler(W94R)-gfpmut3 | This study |
| pGY2195 | pGY1 carrying PLEE1-ler(L95P)-gfpmut3 | This study |
| pGY2212 | pGY1 carrying PLEE1-ler(A98V)-gfpmut3 | This study |
| pGY2196 | pGY1 carrying PLEE1-ler(G102R)-gfpmut3 | This study |
| pGY2213 | pGY1 carrying PLEE1-ler(G102E)-gfpmut3 | This study |
| pGY2214 | pGY1 carrying PLEE1-ler(L109P)-gfpmut3 | This study |
| pGY2501 | pGY1 carrying PLEE1-ler(E4Stop)-gfpmut3 | This study |
| pGY3682 | pGY1 carrying PLEE1-ler(N50Stop)-gfpmut3 | This study |
| pGY3683 | pGY1 carrying PLEE1-ler(G65Stop)-gfpmut3 | This study |
| pSA10 | pKK177-3 derivative containing lacIq | 23 |
| pGY2746 | pSA10 carrying ler | This study |
| pGY2742 | pSA10 carrying ler(L23R) | This study |
| pGY2743 | pSA10 carrying ler(G82E) | This study |
| pGY2744 | pSA10 carrying ler(A98V) | This study |
| pGY2745 | pSA10 carrying ler(G102R) | This study |
| pGY3576 | pSA10 carrying ler(G89D) | This study |
| pQE-70 | Expression of C-terminally His6-tagged proteins | Qiagen |
| pDF5 | pACYC184 containing lacIq and ler expressed from the tac promoter | 12 |
| pDF11 | pQE-70-based, expressing Ler-His6 from the PLEE1promoter | This study |