E. coli strains |
|
|
E2348/69 |
EPEC wild type |
J. Kaper |
TU1403 |
E2348/69 carrying a chromosomal ler-His6
|
3 |
DF2 |
E2348/69 ler::kan
|
12 |
A70 |
CC102 (ara Δ(lac-proB)XIIIthi F′-lacI378 lacZ461 proA+B+) mutS215::Tn10
|
S. Altuvia |
W3110 |
F− λ−thyA36 deoC2 IN(rrnD-rrnE)1
|
A. Oppenheim |
Plasmids |
|
|
pIR1 |
pKK177-3 derivative containing gfpmut3 |
12 |
pGY1 |
pIR1 containing a transcriptional fusion of PLEE1, ler, and gfpmut3 (PLEE1-ler-gfpmut3) |
This study |
pGY2180 |
pGY1 carrying PLEE1-ler(L16P)-gfpmut3 |
This study |
pGY2206 |
pGY1 carrying PLEE1-ler(L23R)-gfpmut3 |
This study |
pGY2189 |
pGY1 carrying PLEE1-ler(L56P)-gfpmut3 |
This study |
pGY2190 |
pGY1 carrying PLEE1-ler(Y77H)-gfpmut3 |
This study |
pGY2191 |
pGY1 carrying PLEE1-ler(Y77C)-gfpmut3 |
This study |
pGY2192 |
pGY1 carrying PLEE1-ler(G82V)-gfpmut3 |
This study |
pGY2207 |
pGY1 carrying PLEE1-ler(G82E)-gfpmut3 |
This study |
pGY2208 |
pGY1 carrying PLEE1-ler(W85R)-gfpmut3 |
This study |
pGY2209 |
pGY1 carrying PLEE1-ler(G87S)-gfpmut3 |
This study |
pGY2210 |
pGY1 carrying PLEE1-lerG87D)-gfpmut3 |
This study |
pGY2211 |
pGY1 carrying PLEE1-ler(G89D)-gfpmut3 |
This study |
pGY2193 |
pGY1 carrying PLEE1-ler(P92S)-gfpmut3 |
This study |
pGY2194 |
pGY1 carrying PLEE1-ler(W94R)-gfpmut3 |
This study |
pGY2195 |
pGY1 carrying PLEE1-ler(L95P)-gfpmut3 |
This study |
pGY2212 |
pGY1 carrying PLEE1-ler(A98V)-gfpmut3 |
This study |
pGY2196 |
pGY1 carrying PLEE1-ler(G102R)-gfpmut3 |
This study |
pGY2213 |
pGY1 carrying PLEE1-ler(G102E)-gfpmut3 |
This study |
pGY2214 |
pGY1 carrying PLEE1-ler(L109P)-gfpmut3 |
This study |
pGY2501 |
pGY1 carrying PLEE1-ler(E4Stop)-gfpmut3 |
This study |
pGY3682 |
pGY1 carrying PLEE1-ler(N50Stop)-gfpmut3 |
This study |
pGY3683 |
pGY1 carrying PLEE1-ler(G65Stop)-gfpmut3 |
This study |
pSA10 |
pKK177-3 derivative containing lacIq
|
23 |
pGY2746 |
pSA10 carrying ler
|
This study |
pGY2742 |
pSA10 carrying ler(L23R) |
This study |
pGY2743 |
pSA10 carrying ler(G82E) |
This study |
pGY2744 |
pSA10 carrying ler(A98V) |
This study |
pGY2745 |
pSA10 carrying ler(G102R) |
This study |
pGY3576 |
pSA10 carrying ler(G89D) |
This study |
pQE-70 |
Expression of C-terminally His6-tagged proteins |
Qiagen |
pDF5 |
pACYC184 containing lacIq and ler expressed from the tac promoter |
12 |
pDF11 |
pQE-70-based, expressing Ler-His6 from the PLEE1promoter |
This study |