FIG. 5.
Effect of mutation of hetF and hetR on transcription from the −271 TSP of hetR and transcription from the hetR and patS promoters. Agarose gel electrophoresis of RACE products from the −271 and −184 (left panel) and −728 and −696 (right panel) TSPs is shown in panel A; lanes are as indicated. Also shown is light (upper panel) and fluorescence (lower panel) microscopy of a mixture of filaments of Anabaena sp. strains PCC 7120 and UHM103, which is a ΔhetR mutant; strain 216, which carries the hetR(S179R) allele, or UHM130, which is a ΔhetF mutant carrying pSMC127, a plasmid bearing PhetR-gfp (B), or pAM1951, a plasmid bearing PpatS-gfp (C). Strain designations are indicated by arrows.