TABLE 2.
Oligonucleotides used in this study
| Oligonucleotidea | Sequence |
|---|---|
| hetF-NdeI-F | CATATGAATGACCCATTGAAATCAG |
| hetF-H6-BamHI-R | ATATAGGATCCCTAGTGATGGTGATGGTGATGCTTGGGGCTTTTTTGTTG |
| hetF-Up-F | ATATAGGATCCAGTCGGTGTTAGCCAGCTAG |
| hetF-Up-R | GATTTCCGCGGGTCATTCATCAAATGCCCAG |
| hetF-Down-F | AACAACCGCGGCCCAAGTAGAGTACGGGAAG |
| hetF-Down-R | TTGCAGAGCTCTGACCTAAAGCCGCCATTTTC |
| hetF-BamHI-R | ATATAGGATCCCTACTTGGGGCTTTTTTGTTG |
| hetFH201Y-F | CTACTCCGGTTATAGCAATTTAGGCGCGAACG |
| hetFH201Y-R | CTAAATTGCTATAACCGGAGTAGTGTAAAACATG |
| hetFC246A-F | GTTTAACTCCGCGTGGGGTACCTACACCGCGAG |
| hetFC246A-R | GGTACCCCACGCGGAGTTAAACACCGCCATTTG |
| TNL-GFP-F | TTTGGATCCAATCCCGGGGATCGGCGTCAGCTAGTAAAGGAGAAGAACTTTTCACTGGA |
| TNL-GFP-R | ACAGAGCTCTTATTTGTATAGTTCATCCATGCCATG |
| PhetF-SacI-F | ATATAGAGCTCTGTAGCTCTGTGTCTCTTGTTG |
| PhetF-SmaI-R | ATATACCCGGGCCGCCAACCAATCAGCTAC |
| PpetE-BamHI-F | ATATAGGATCCCTGAGGTACTGAGTACACAG |
| PpetE-SmaI-R | ATATACCCGGGACCTGTAGTTTTATTTTTCTTATTTC |
| Pnir-SmaI-R | ATATACCCGGGAAGTTTTTTTGCTCAAGATCAATCC |
| Pnir-BamHI-F | GCGCGCGGATCCAGCTACTCATTAGTTAAGTGTAATG |
| PhetF-BamHI-F | ATATAGGATCCTGTAGCTCTGTGTCTCTTG |
| hetR-SmaI-R | ATATACCCGGGAATCTTCTTTTCTACCAAACACC |
| nir1-SacI | GCGCGCGAGCTCAGCTACTCATTAGTTAAGTGTAATG |
| hetRcf-NdeI | CATATGAGTAACGACATCGATCTGATC |
| hetR6H-r | TTAGTGATGGTGATGGTGATGATCTTCTTTTCTACCAAACACCATTTG |
| hetRH6-SacI-R | TTACTGAGCTCTTAGTGATGGTGATGGTGATGATCTTCTTTTCTACCAAACACCATTTG |
| hetRcr-SacI | GAGCTCTTAATCTTCTTTTCTACCAAACACC |
| PpetEF-SacI | TTTGGATCCTGCAGTCGGTAATTTGGTGTTTTTCC |
| PhetF-Seq-1 | AAATTGCATCAAAGGTATGCTG |
| hetF-con-R | GTATGGACAAGATTACCAGC |
| 696newII | AATCGCACAATCTCTCCTTGAGGTG |
| 184newI | CATTGCACTGGGGCCAAGACGCTT |
| 696newI | TTTGCTCTTATGGCAGTGTAGGTCG |
| New184nesrev | AAAGTAGTTGGTATACACCCATAGGGGG |
| PpetE-F | GCTGAGGTACTGAGTACACAGCTAAT |
| PpetE-gfp-R | TTCTCCTTTACTCATGGCGTTCTCCTAACCTGTAG |
| Txp-LacZ-F | CGCGGATCCATCCCGGGAGGAAACAGCTATGACCATGATTAC |
| Txp-LacZ-R | CGCGGTACCTTATTTTTGACACCAGACCAACTGG |
| gfp-PpetE-F | TAGGAGAACGCCATGAGTAAAGGAGAAGAACTTTTCACTG |
| gfpKPNI-R | GGTACCTTATTTGTACAATTCATCCATACC |
a
Oligonucleotides are shown in the 5′-to-3′ direction.