FIG. 6.

Mutations at a single pseudouridylation site are not sufficient to relieve the cold sensitivity of the ΔbipA strain. KKQ113 (ΔbipA in the six-rrn deletion strain) was transformed with single-site mutant plasmids, and analysis of growth rates at 20°C was performed. pRrnB was the parent plasmid carrying wild-type rrnB and promoted cold-sensitive growth. All assays were performed in triplicate. (A) The change of residue U955 to adenine (in pRrnB-U955A) or guanine (in pRrnB-U955G) did not abolish cold sensitivity. (B) The alteration of nucleotide U2580 to adenine (in pRrnB-U2580A), cytosine (in pRrnB-U2580C), or guanine (in pRrnB-U2580G) also did not abolish the cold sensitivity.