FIG. 1.

Engineering nsp2 deletions, mutations, rearrangements, and duplications. For each construct, alterations to the genome are shown. Constructs are listed as named in the text. ORFs 1 to 7 are labeled above the wild-type schematic. The nsp2 coding sequence is depicted as a hatched rectangle. Coding region locations and sizes are not drawn to scale. Deletion of the nsp2 and/or ORF4 coding sequence or insertion of nsp2 in ORF1b is indicated by a caret. Protein coding deletions are indicated by a delta (Δ) in the virus name. The nsp2 position is indicated as ORF1a (1a-2), ORF1b (13-2-14 or 10-2-12), or ORF4 (4-2). Approximate peak titers of viable viruses are indicated to the right of each construct. Viruses that were not recovered are indicated by a minus sign (−).