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Free-zinc ions in keratinocytes. Keratinocytes with a mutated EVER gene (EVER2−/−) were loaded with Zinquin, a fluorescent indicator specific for free zinc; subsequently, the cells were incubated for 15 min in 100 μM zinc. A strong fluorescence, corresponding to places with a high concentration of free zinc, is visible mainly in the nucleoli (indicated by red arrows).
