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Changes related to autophagy in CVB4-infected neurons. (A) Western blotting shows that levels of LC3-II, VP1, and the calpain-cleaved spectrin fragment increase in CVB4-infected neurons but decrease in 3-MA-treated neurons. *, P < 0.01 versus the result for CVB4-infected untreated neurons. (B) The assay of progeny virus production and a sequential plaque assay showed that 3-MA decreases virus replication. *, P < 0.01.
