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HIV-1-restricting ability of TRIM5-Cyp A fusion proteins with changes in the B-box 2 domain. HeLa cells stably expressing wild-type TRIM5αrh or rh5RBCC-Cyp B-box 2 mutants, or cells transduced with the empty LPCX vector, were exposed to increasing amounts of HIV-1-GFP. Infected, GFP-positive cells were counted by FACS. The results of a single experiment are shown; similar results were obtained in a repeat experiment.
