TABLE 1.

Parameters providing the best fit of the model with preparation-induced death of targets (given in equations 5 and 6) to the data^a

Parameter	Mean	Low 95% CI	High 95% CI
ɛ, min−1	4.71 × 10−3	3.34 × 10−3	6.17 × 10−3
αA, min−1	7.17 × 10−12	5.58 × 10−12	9.22 × 10−12
αM, min−1	1.30 × 10−11	0.92 × 10−11	1.89 × 10−11
(min−1)	3.45 × 10−1	2.59 × 10−1	4.56 × 10−1
(min−1)	5.0 × 10−2	3.80 × 10−2	6.41 × 10−2
(min−1)	1.44 × 10−2	1.10 × 10−2	1.82 × 10−2
(min−1)	4.15 × 10−3	3.21 × 10−3	5.52 × 10−3

^aHere α_A and α_M are coefficients relating the recruitment rate of cells into the spleen in acutely infected (σ = α_AN_s) or LCMV-immune (σ = α_M N_s) mice, and the number of splenocytes in individual mice is N_s. In the fits, the rate of migration of labeled splenocytes to other organs, δ, was fixed to 0 since this did not affect the quality of the model fit to data (F_1,190 = 0.15; P = 0.68). Data and model fits are shown in Fig. 2. The death rate of targets estimated by the model for acutely infected mice is as follows: = 497 and = 72 per day for NP396- and GP276-pulsed targets, respectively. In memory mice, the death rate of peptide-pulsed targets is as follows: = 21 and = 6 per day for NP396- and GP276-pulsed targets, respectively. These rates correspond to half-lives of 2 and 14 min for NP396- and GP276-expressing targets at the peak of the CD8 T-cell response and 48 min and 2.8 h for NP396- and GP276-expressing targets in LCMV-immune mice, respectively. CIs were calculated by bootstrapping the data with 1,000 simulations (19).