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Reactivation of expression at 38.5°C. HFFF2 monolayers containing quiescent in1374 were left untreated (control), incubated with 50 μM sodium arsenite (As) for 16 h at 38.5°C, incubated at 44°C for 45 min (heat shock), incubated with 8 μg/ml or 12 μg/ml gramicidin D (Gm) for 16 h at 38.5°C, or infected with 3 PFU of tsK per cell. After treatment, cells were incubated at 38.5°C for 20 h and stained for the presence of β-galactosidase.
