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. 2008 Sep 17;82(23):11555–11567. doi: 10.1128/JVI.01561-08

FIG. 4.

FIG. 4.

LEDGF/p75 mutant protein activities and expression profiles in E2(−/−) knockout cells. (A) The indicated mutants were tested in comparison to wild-type LEDGF/p75 and empty pIRES2-eGFP vector DNA; data are averages and standard deviations of minimally eight Luc assays from two or more independent transfection and infection experiments. (B) Expression levels of proteins analyzed in panel A. Lanes 1 and 2, endogenous mouse LEDGF/p75 content of parental E1(f/+) and E2(−/−) cells, respectively; HIV-Luc infected these knockout cells at 3.6% ± 0.8% (n = 3) of the level of E1(f/+) cells. (C) Levels of wild-type LEDGF/p75 and ΔPWWP proteins in transiently transfected E2(−/−) (lanes 1 and 2) and E6(−/−) (lanes 5 and 6) cells, respectively. Lanes 3 and 4, endogenous mouse LEDGF/p75 levels in E2(−/−) and E1(f/+) cells, respectively, transfected with empty vector DNA; lanes 7 and 8, endogenous protein levels in transfected E6(−/−) and E6(f/f) cells, respectively.