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Wild-type and mutant LEDGF/p75 association with chromatin as assessed by biochemical fractionation. (A) Fractionation scheme (adapted from reference 29). (B) Fractionation profiles of 293T cells transfected with plasmids expressing the indicated HA-tagged proteins. Similar results were obtained using E6(−/−) knockout cells. (C and D) Same as in panel B, except with the indicated mutant proteins.
