FIG. 8.

PWWP domain DNA and chromatin binding activities. (A) The upper panel shows naked DNA levels recovered via GST pull-down with the indicated proteins. The middle panels show PN pull-downs as assessed by autoradiography (DNA) and Western blotting with anti-histone H3 antibodies. The lower panel shows bound fractions of the different GST proteins. WT, wild-type. (B) Results of four to six independent sets of pull-down assays, expressed as the percent DNA recovery with respect to the wild-type PWWP domain ± the standard deviation. Naked DNA experiments are indicated by black bars, whereas gray bars show the results with PNs. *, P < 0.01 for the differences observed between DNA and chromatin binding using Student's t test.