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. 2008 Sep 24;82(23):11705–11713. doi: 10.1128/JVI.01320-08

FIG. 1.

FIG. 1.

SR inhibitors reduce the uptake of Ad by KCs and enhance liver transduction. (A) C57BL/6 mice were injected i.v. with 0.2 mg of SR inhibitors or noninhibitors in water. Poly(I), poly(G), and dextran sulfate are broad-specificity SR inhibitors, while poly(A) and heparin inhibit a more limited subset of SRs. Five minutes later, mice were injected i.v. with 1.0 × 1011 vp/kg of Av1nBg. Mice were sacrificed 10 min after Ad injection, and the amount of Ad (intensity of immunofluorescent staining) was quantitated specifically within KCs. Three to five mice per group. *, P value of <0.05 versus the control water-predosed group (Holm-Sidak post hoc). (B) SR inhibitors enhanced liver transduction by Ad. C57BL/6 mice were injected i.v. with 0.2 mg of SR inhibitors [poly(I) and dextran sulfate] or an SR noninhibitor [poly(C)]. After 5 min, mice were injected i.v. with 5.0 × 1011 vp/kg of Av1nBg. After an additional 48 h, mice were sacrificed and the amount of β-galactosidase in the liver was quantitated. Four mice per group. *, P value of <0.05 versus control water-preinjected mice (Holm-Sidak post hoc). RLU, relative light units.