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. 2008 Sep 17;82(23):11979–11984. doi: 10.1128/JVI.00867-08

FIG. 1.

FIG. 1.

Small plaque formed by the Edmonston strain and recombinant MVs with the P or L gene of the Edmonston strain on Vero/hSLAM cells. (A) Plaque assays were performed for various MV strains (three wild-type strains [IC323, JPN/50.98/1, and JPN/26.99/1] and two Edmonston lineage strains [Ed-ATCC and Ed-tag]). Monolayers of Vero/hSLAM cells on 12-well cluster plates were infected with 50 PFU of each virus and overlaid with Dulbecco's modified Eagle medium containing 2% fetal bovine serum and 1% methylcellulose. At 5 days p.i., the cells were stained with RTU Vectastain Elite ABC reagent (Vector Laboratories) using anti-MV H-protein monoclonal antibodies and a biotinylated secondary antibody. After high-resolution digital images were obtained, the sizes of all plaques were measured. The mean sizes ± standard deviations are shown in the bar graph. (B) Plaque assays were performed for wild-type IC323-EGFP and six recombinant MVs (each containing one of the Ed-tag strain genes in the backbone of the IC323-EGFP genome) on Vero/hSLAM cells, as described for panel A.