FIG. 3.
Neutral effects of substitutions in the leader region of the Edmonston strain on MV gene expression levels and plaque sizes. (A) Confluent monolayers of CV1/hSLAM, HeLa/hSLAM, and CHO/hSLAM cells cultured in 24-well cluster plates were infected with 2.5 × 103 PFU of IC323-Luci (circles) and IC/Ed-Le-Luci (triangles). After various intervals, the Renilla luciferase activities were measured. Data are means ± standard deviations for triplicate samples. RLU, relative light units. (B) Plaque assays were performed for the recombinant MVs, as described for Fig. 1A. The IC/Ed-Le-Luci, IC/Ed-LeP-Luci, IC/Ed-LeL-Luci, and IC/Ed-LePL-Luci genomes contain Ed-tag genome regions encoding Le alone, Le and P, Le and L, or Le, P, and L, respectively, in the backbone of the IC323-Luci genome.