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. 2008 Sep 24;82(23):11837–11850. doi: 10.1128/JVI.01623-08

FIG. 4.

FIG. 4.

ARPE-19 cells fuse when gB and gH/gL are transduced into all of the cells in a monolayer. Two conditions for transduction of ARPE-19 cells were compared. In some instances, ARPE-19 cells were transduced with Ad vectors expressing gB, gH, and gL (A) or gB, gH, gL, and UL128-131 (C) and then mixed with untransduced ARPE-19 cells (mixed). In other cases, Ad vectors expressing gB, gH, and gL (B) or gB, gH, gL, and UL128-131 (D) were used to directly transduce monolayers of ARPE-19 cells (monolayers). Transductions of gB, gH, and gL were performed at 25 PFU/cell. To attain similar levels of expression, transductions involving gB, gH, gL, and UL128-131 involved higher doses (75 PFU/cell). (E) Quantification of cell-cell fusion under the conditions in panels A to D by the methods described in the legend to Fig. 1. Values represent the averages derived from three separate images, and the error bars indicate the standard deviations.