TABLE 1.
Sense sequence of oligonucleotides used for EMSA analysis
Oligonucleotide | Position | Sequence (5′-3′)a |
---|---|---|
Minimal TRE | −59 to +1 | TCGAGCGTGGTGACGTCGCAACGCGGCGCAGGGTGAGAGCGCGCGCTTGCGGACGCGGCGGCAT |
Distal | −48 to −30 | CTAGAGTCGCAACGCGGCGCAGGGA |
Proximal | −21 to −1 | CTAGACGCGCTTGCGGACGCGGCGGCT |
Δ PIR | −21 to −1 | CTAGAACATCTTGCGGACGCGGCGGCT |
Δ PDR | −21 to −1 | CTAGACGCGCTTGCGATCTGGGCGGCT' |
Sequences in bold identify the positions of the inverted and direct repeats. Underlined sequences identify the positions of mutations.