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. 2008 Sep 17;82(23):11682–11694. doi: 10.1128/JVI.01562-08

FIG. 6.

FIG. 6.

Electron microscopic images of CA-NC assemblies incubated with full-length TRIM5-21R or deletion mutants. Negatively stained, transmission electron microscopic images of CA-NC assemblies incubated with monomeric TRIM5-21RΔSPRY (panels 1 and 2), dimeric TRIM5-21RΔV1 (panels 3 and 4), or full-length dimeric TRIM5-21R (panels 5 to 8). Note that CA-NC/DNA assemblies incubated with TRIM5-21R show enhanced exterior staining and more frequent breaks (arrows) compared to the TRIM5-21RΔV1 or TRIM5-21RΔSPRY controls. TRIM5-21R-induced breaks were quantified by scoring randomly selected electron microscopic images of CA-NC/DNA tubes for the presence of discontinuities (see arrows), with the following results: TRIM5-21RΔSPRY-treated tubes (0% broken, 0 total breaks, n = 26 tubes), TRIM5-21RΔV1-treated tubes (4% broken, 2 total breaks, n = 50), and TRIM5-21R-treated tubes (42% broken, 55 total breaks, n = 50).