Skip to main content
. Author manuscript; available in PMC: 2009 Aug 2.
Published in final edited form as: DNA Repair (Amst). 2008 Jun 26;7(8):1340–1351. doi: 10.1016/j.dnarep.2008.04.018

Figure 5.

Figure 5

NHEJ activity of Pol λ BRCT domain mutants. (A). Pol λ (PDB code 2JW5), blue) superimposed with Pol µ (PDB code 2DUN, maroon). Residues in α-helix 1 crucial for interaction with core NHEJ factors in Pol µ (R43, F46, and L50, light red) are shown with sticks, relative to tested positions in Pol λ (R55, R57, L60 and Q64, light blue). Secondary structural elements of Pol λ BRCT are shown in the background in light gray. (B). EMSA analysis was performed in the presence of a 60 bp DNA duplex, Ku and the XRCC4-ligase IV (X4-LIV) complex and various full-length Pol λ proteins. . The composition of each species of distinct mobility is noted with cartoons at the left of the panel. C) Joining of a 300 bp substrate with three nucleotide AGC 3’ overhangs was performed in the presence of Ku, XRCC4-Ligase IV, and various full-length Pol λ constructs as noted. S, substrate, P, joined concatemer products.