Figure 3.

Fine specificity analysis of mutant scTCR binding to different QL9 variant peptides bound to L^d. The original T cell clone 2C and various yeast clones were analyzed by flow cytometry for binding to L^d/Ig dimers loaded with wild-type QL9 (QL9–5F), position 5 variants of QL9 (QL9–5Y, QL9–5H, and QL9–5E) or MCMV. Binding was detected with FITC-labeled goat anti-mouse IgG. Relative fluorescence was measured by comparison with mean fluorescence values of 2C cells or yeast cells stained with anti-Vβ8 antibody F23.2.