Figure 4.
QL9/Ld binding by soluble scTCRs. T2-Ld cells loaded with QL9 were incubated with 125I-labeled anti-Ld Fab fragments (30–5-7) and various concentrations of unlabeled Fab (□), scTCR-T7 (■), or mutant scTCR-m6 (●). Bound and unbound [125I]30–5-7 Fab fragments were separated by centrifugation through olive oil/dibutyl phthalate. Binding of 125I-labeled anti-Ld Fab fragments to T2-Ld cells loaded with the control peptide MCMV was not inhibited even at the highest concentrations of scTCRs (data not shown).