Table 3.

Epitope of muscarinic receptor antisera used

Receptor	Epitope sequence	Corresponding residues	Fusion protein
MR1	GSETPGKGGGSSSSSERSQPGAEGSPETPPGRCCRCCRAPRLLQAYSWKEEEEEDEGSMESLTSSEGEEPGSEVVIKMPMVDPEAQAPTKQPPRSSPNTVKRPTKKGRDRAGKGQKPRGKEQLAKRK	AA 227-353 of human MR1	GST
MR2	VANQDPVSPSLVQGRIVKPNNNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSNDSTSVSAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKSDSCTPTNTTVEVVGSSGQNGDE	AA 225-356 of human MR2	GST
MR3 (R&D)	FHKRVPEQAL	AA 580-589 of rat MR3	KLH
MR3 (Alomone)	TLAKRFALKTRSQITKRKR	AA 461-479 rat MR3	mcKLH
MR4	Third intracellular loop of human MR4	Sequence details not disclosed by company	GST
MR5	EEKLYWQGNSKLP	AA 519-531 of rat MR5	KLH

MR, muscarinic receptor; GST, gluthathione S-transferase; KLH, keyhole limpet hemocyanin; mcKLH, maleimide-activated keyhole limpet hemocyanin.