Light micrographs of efferent ducts (EDs; columns 1 and 3) and initial segment of the epididymis (IS; columns 2 and 4) in young rats (Y; columns 1 and 2) and adult rats (A; columns 3 and 4) in zinc-fixed tissues immunostained for AQP 5 (A–D), AQP 7 (E–H), AQP 9 (I–L), and AQP 11 (M–P) by fluorescence method. Differences in the intensities of immunostaining between young and adult rats are evident in EDs for AQP 7 and 11 and in IS for AQP 9. E, epithelium; IT, intertubular space; Lu, lumen; Mv, microvilli; n, nuclei; P, principal cells; arrows, weak reactions along the base of the epithelium; circle, reactive sperm. Bars: A–P = 40 μm; inset = 10 μm.