Table 1.
RT-PCR primers for EDs and Epi in adult rats
| Detected in
|
|||||
|---|---|---|---|---|---|
| Gene | Primer sequence (forward/reverse) | Predicted fragment size (bp) | Tested (this study) | EDs | Epi |
| AQP 0 | CCCCACCAGCTGTCCGAGGAAACC | 248 | X | ||
| GAGCAAAGGAGCGGGCGGGATTC | |||||
| AQP 1 | TGGCCAGCGAAATCAAGAAGA | 249 | √ | √ | √ |
| GAGGATGGCGGAGGCAACGAT | |||||
| AQP 2 | GCCATCCTCCATGAGATTACTCCA | 323 | √ | X | X |
| CAGGGGTCCGATCCAGAAGA | |||||
| AQP 3 | CTTCGCTGTCACCTTGCCATCTT | 228 | √ | X | √ |
| GGGCCGGAGACAACAAGCTCATT | |||||
| AQP 4 | CTGGCCATGCTCATCTTTGTTC | 821 | √ | X | X |
| AGACGAGTCCTTCCCCTTCTTCTC | |||||
| AQP 5 | TCTTCTTTGGCCTGGGCTCAGCAC | 388 | √ | X | √ |
| CGGCGAGAGTCGGTGGAGGAGA | |||||
| AQP 6 | CTACAGTTGGGGCTGCTCTTCTTT | 397 | √ | X | X |
| GGCTACGGTCTTGGTGTCAGG | |||||
| AQP 7 | GGCAGGCGGCATCTCTGGAG | 397 | √ | √ | √ |
| GGACACCCCAAGAACGCAAACAAG | |||||
| AQP 8 | CTTGGGGCTCATCATTTGCTACTT | 330 | √ | X | X |
| AGGGGACCCATTGTCTTCTCATTG | |||||
| AQP 9 | ATCGCAGTCGTGATGGCTCTCTA | 296 | √ | √ | √ |
| CTACAAAGGCACCTGGCGTGGAT | |||||
| AQP 10 | X | ||||
| AQP 11 | X | √a | |||
| AQP 12 | X | ||||
a
As reported by Da Silva et al. 2006b.
EDs, efferent ducts; Epi, epididymis; AQP, aquaporin.