Figure 3. 3D HCCH-COSY spectrum.

A heteronuclear 3D NMR spectrum of crude ¹³C-labeled T87 cultured cell extracts. 2D ¹H–¹H planes at 57.702, 62.702, 72.077, and 77.701 ppm of ¹³C cut through the 3D HCCH-COSY spectrum are shown (for each spectrum, the relative position of each cut is shown in a 3D box at the upper left corner). Red lines connect detected cross peaks of the named metabolites. In total, 89 HSQC peaks corresponding to 24 metabolites were confirmed. The spectrum was acquired with 1024 points per 14 ppm in the ¹H direct dimension, accumulating 16 transients per free induction decay, 100 increments per 40 ppm in the ¹³C dimension, and 48 increments per 8 ppm in the indirect ¹H dimension.